T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.
IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Interleukin-2 Human Recombinant produced in HEK cells is a glycosylated monomer, having a total molecular weight of 15kDa.
The IL2 is purified by proprietary chromatographic techniques.
Sterile Filtered White lyophilized (freeze-dried) powder.
The IL2 was lyophilized from 0.2µm filtered solution containing 1mg/ml protein in 1xPBS.
It is recommended to reconstitute the lyophilized IL-2 in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
Lyophilized IL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Greater than 95% as obsereved by SDS-PAGE.
The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line) and is typically 0.2-2ng/ml.
Safety Data Sheet
Amino acid sequence
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.