prospec

IL-2 Canine

  • Name
  • Description
  • Pricings
  • Quantity
  • IL-2 Canine
  • Interleukin-2 Canine Recombinant
  • Shipped at Room temp.

Catalogue number

CYT-1096

Synonyms

Interleukin-2, IL-2, T-cell growth factor, TCGF.

Introduction

IL2 is a secreted cytokine that is essential for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 and interleukin 7. The expression of IL2in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the exact expression of a single gene.

Description

Interleukin-2 Canine Recombinant produced in E. coli is a non-glycosylated monomer chain containing 136 amino acids and having a molecular mass of 15.6kDa.

IL-2 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a sterile (0.2µm) filtered solution containing 20 mM sodium bicarbonate, pH 8.5.

Solubility

It is recommended to reconstitute the lyophilized IL-2 in Sterile 10 mM sodium bicarbonate, pH 8.5 at 0.1 mg/mL, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized IL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-2 should be stored at 4°C between 2-7 days and for future use below -18°C.

Please prevent freeze-thaw cycles.

Amino acid sequence

MAPITSSSTK ETEQQMEQLL LDLQLLLNGV NNYENPQLSR MLTFKFYTPK KATEFTHLQC LAEELKNLEE VLGLPQSKNV HLTDTKELIS NMNVTLLKLK GSETSYNCEY DDETATITEF LNKWITFSQS IFSTLT.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Biological Activity

The ED50, as determined by CTLL-2 cell proliferation is <5ng/ml corresponding to a specific activity which is ≥ 2.0 x 10^5 units/mg.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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