- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
CYT-382
Synonyms
T-cell growth factor (TCGF), Interleukin-2, Lymphokine, IL-2.
Introduction
Description
Interleukin-2 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 16kDa.
The IL-2 is purified by proprietary chromatographic techniques.
Source
Physical Appearance
Sterile filtered colorless solution.
Formulation
The IL-2 solution contains 0.2µm filtered solution in 20mM PB pH3.5 and 30% Glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 97.0% as determined by SDS-PAGE and HPLC.
Amino acid sequence
APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ.
Biological Activity
The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is less than 0.2ng/ml, corresponding to a Specific Activity of 5,000,000IU/mg.
References
Title:Functional Gap Junctions Facilitate Melanoma Antigen Transfer and Cross-Presentation between Human Dendritic Cells.
Publication:The Journal of Immunology June 1, 2007 vol. 178 no. 11 6949-6957
Link:IL 2 Rat prospec publication
Safety Data Sheet
Usage
Background
Title: Interleukin-2 Rat Recombinant: A Key Cytokine for Immunological Research
Abstract:
Interleukin-2 (IL-2) is a critical cytokine that plays a pivotal role in immune responses. This research paper provides a comprehensive analysis of rat recombinant IL-2, focusing on its production, purification, and applications in immunological research. The paper discusses the significance of IL-2 in immune cell proliferation, activation, and regulatory functions. Furthermore, it elucidates the potential therapeutic implications of recombinant IL-2 in immunotherapy and highlights ongoing research in the field. The information presented in this paper aims to enhance the understanding of rat recombinant IL-2 and its utility as a research tool in immunological studies.
Introduction:
Interleukin-2 (IL-2) is a cytokine that plays a crucial role in immune regulation, particularly in T cell activation and proliferation. Rat recombinant IL-2, produced through genetic engineering techniques, enables researchers to investigate its biological functions and therapeutic potential.
Production and Purification:
Recombinant IL-2 is typically produced using expression systems such as bacteria, yeast, or mammalian cells. The protein is then purified using various chromatographic techniques to obtain a highly pure and biologically active form. Quality control measures ensure the integrity and functional activity of the recombinant protein.
Immunological Applications:
Rat recombinant IL-2 has extensive applications in immunological research. It serves as a potent growth factor for T cells, promoting their proliferation and differentiation. IL-2 also plays a critical role in the development and maintenance of regulatory T cells, which are essential for immune tolerance and homeostasis. Recombinant IL-2 is a valuable tool for studying these processes and dissecting the underlying mechanisms.
Therapeutic Implications:
The immunomodulatory properties of IL-2 have led to its exploration as a potential therapeutic agent in immunotherapy. Recombinant IL-2 has been investigated for its use in cancer immunotherapy, autoimmune disorders, and infectious diseases. Ongoing research focuses on optimizing IL-2-based therapies, including combination treatments and targeted delivery approaches.
Conclusion:
Rat recombinant IL-2 is a fundamental research tool in the field of immunology. Its production, purification, and applications in immune cell proliferation and regulation contribute to our understanding of immune responses and the development of novel therapeutics. Continued research and advancements in IL-2-based immunotherapy hold promise for improving treatment outcomes in various immunological disorders.
References
Bibliography:
- Rosenberg, S. A., & IL-2: The First Effective Immunotherapy for Human Cancer. Journal of Immunology, 192(12), 5451-5458.
- Boyman, O., & Sprent, J. (2012). The role of interleukin-2 during homeostasis and activation of the immune system. Nature Reviews Immunology, 12(3), 180-190.
- Malek, T. R. (2008). The biology of interleukin-2. Annual Review of Immunology, 26, 453-479.
- Smith, K. A. (1988). Interleukin-2: inception, impact, and implications. Science, 240(4856), 1169-1176.
- Nelson, B. H. (2004). IL-2, regulatory T cells, and tolerance. Journal of Immunology, 172(7), 3983-3988.