- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
ANT-102
Synonyms
T-cell growth factor (TCGF), Interleukin-2, Lymphokine, IL-2.
Type
Mouse Anti Human Monoclonal.
Introduction
IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Clone
YNRhIL2.
Immunogen
r.Human IL-2.
Ig Subclass
Mouse IgG2a.
Purification Method
Ion Exchange.
Formulation
1mg/ml in PBS (after reconstitution).
Shipping Conditions
Antibody is shipped lyophilized at ambient temperature.
Storage Procedures
In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
Solubility
Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Titer
By direct ELISA: 1:10,000 dilution will yield 0.4 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). 1:50 dilution will block 70-80% of CTLL proliferative response to 50 units of r.human IL-2.
Applications
Direct ELISA, Western Blot, Immuneprecipitation, immunohistochemistry, blocking T cell poroliferation.
Cross reactivity
A certain degree of functional cross reactivity with murine IL-2 was reported by some investigators.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
Title: Interleukin-2, Mouse Anti-Human: A Versatile Tool for Immunological Research
Abstract:
Interleukin-2 (IL-2) is a key cytokine involved in the regulation of immune responses. Mouse anti-human IL-2 antibodies have emerged as valuable tools in immunological research, enabling the detection, quantification, and functional analysis of IL-2 in various experimental settings. This research paper provides an overview of mouse anti-human IL-2 antibodies, their applications in immunological research, and their significance in advancing our understanding of immune system function.
Introduction:
IL-2 plays a pivotal role in immune regulation, serving as a critical growth factor for T cells and natural killer (NK) cells. The detection and measurement of IL-2 are essential for studying immune responses in various contexts, including infectious diseases, autoimmunity, and cancer. Mouse anti-human IL-2 antibodies have proven to be indispensable tools for researchers, enabling the specific and sensitive detection of IL-2 in experimental systems.
Characteristics and Applications:
Mouse anti-human IL-2 antibodies are monoclonal antibodies derived from mice that specifically recognize and bind to IL-2 molecules. These antibodies can be used in various applications, including enzyme-linked immunosorbent assays (ELISAs), flow cytometry, Western blotting, immunohistochemistry, and in vivo functional studies. They allow researchers to quantify IL-2 levels, identify IL-2-producing cells, and investigate the role of IL-2 in immune cell activation, differentiation, and effector functions.
Significance in Immunological Research:
Mouse anti-human IL-2 antibodies have significantly contributed to our understanding of immune system function. They have been instrumental in elucidating the role of IL-2 in T cell proliferation, survival, and tolerance. These antibodies have also facilitated studies on the effects of IL-2 in modulating the immune response against pathogens, shaping the development of regulatory T cells, and enhancing the efficacy of immunotherapies for cancer treatment.
Future Perspectives:
Continued research and development of mouse anti-human IL-2 antibodies are essential for advancing immunological research. Efforts should focus on generating antibodies with improved sensitivity, specificity, and versatility. Furthermore, the development of antibody-based therapeutics targeting IL-2 holds promise for the treatment of immune-related disorders and cancer.
Conclusion:
Mouse anti-human IL-2 antibodies have become indispensable tools in immunological research, providing valuable insights into the role of IL-2 in immune responses. Their applications in detection, quantification, and functional analysis of IL-2 have expanded our understanding of immune system regulation and paved the way for the development of novel immunotherapeutic strategies. Continued advancements in antibody technology will further enhance our ability to investigate the complex interactions between IL-2 and the immune system, ultimately leading to improved clinical outcomes.
References
Bibliography:
- Boyman O, Sprent J. The role of interleukin-2 during homeostasis and activation of the immune system. Nat Rev Immunol. 2012;12(3):180-190.
- Nelson BH. IL-2, regulatory T cells, and tolerance. J Immunol. 2004;172(7):3983-3988.
- Waldmann TA. The biology of interleukin-2 and interleukin-15: implications for cancer therapy and vaccine design. Nat Rev Immunol. 2006;6(8):595-601.
- Gaffen SL, Liu KD. Overview of interleukin-2 function, production and clinical applications. Cytokine. 2004;28(3):109-123.
- Malek TR, Bayer AL. Tolerance, not immunity, crucially depends on IL-2. Nat Rev Immunol. 2004;4(9):665-674.