Recombinant Porcine Trypsin is expressed in E.coli and purified by standard chromatography techniques.
Sterile Filtered lyophilized powder.
The Porcine Trypsin was lyophilized with mannitol as preservative.
It is recommended to reconstitute the lyophilized Porcine Trypsin in sterile 1mM HCl or 50mM HAC not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Recombinant Porcine Trypsin although stable at room temp for 1 week, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
4500 USP units/mg protein.
One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.0ml at pH7.6 and 25°C, with BAEE as a substrate (1cm light path).
Trypsin digestion: the suggested ratio is 1:50 to 1:1000 (w/w).
Amino acid sequence