prospec

Trypsin-1 Human

  • Name
  • Description
  • Pricings
  • Quantity
  • Trypsin-1 Human
  • Trypsin-1 Human Recombinant
  • Shipped with Ice Packs

Catalogue number

PRO-2049

Synonyms

Protease Serine 1 (Trypsin 1), TRY1, Cationic Trypsinogen,  TRP1, TRYP1, TRY4, beta-trypsin, Digestive Zymogen, Nonfunctional Trypsin 1, Trypsinogen 1, Trypsinogen A, EC 3.4.21.4.

Introduction

Trypsin-1, which belongs to the trypsin family of serine proteases, is localized to the T cell receptor beta locus on chromosome 7. Trypsin-1 is secreted by the pancreas and cleaved to its active form in the small intestine. Trypsin-1 operates on peptide linkages involving the carboxyl group of lysine or arginine. Alterations are the cause for hereditary pancreatitis. 

Description

Trypsin-1 Human Recombinant produced in CHO cells is a 24.1kDa single polypeptide chain consisting of 224 amino acids.

Source

Chinese Hamster Ovary cells.

Physical Appearance

Sterile filtered colorless solution.

Formulation

The Trypsin-1 filtered 0.22um solution contains 20mM TRIS-HCl buffer pH 7.2.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. 
Store, frozen at -20°C for longer periods of time. 
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95% as determined by SDS-PAGE.

Amino acid sequence

IVGGYNCEEN SVPYQVSLNS GYHFCGGSLI NEQWVVSAGH CYKSRIQVRL GEHNIEVLEG NEQFINAAKI IRHPQYDRKT LNNDIMLIKL SSRAVINARV STISLPTAPP ATGTKCLISG WGNTASSGAD YPDELQCLDA PVLSQAKCEA SYPGKITSNM FCVGFLEGGK DSCQGDSGGP VVCNGQLQGV VSWGDGCAQK NKPGVYTKVY NYVKWIKNTI AANS.

Biological Activity

1 unit hydrolyzes 1 µmole of Na-Benzoyl-D,L-arginine 4-nitroanilide (BANI) per minute at 37°C, pH 8.0, in the presence of 10mM calcium.
The specific activity is >1,000 Units per mg protein as measured under the described conditions. 

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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