- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
PRO-283
Introduction
Description
Source
Physical Appearance
Formulation
The Streptavidin was lyophilized from a 25mg/ml solution in 10 mM potassium phosphate buffer pH 6.5
Solubility
Stability
For longer storage in dissolved form add 1mM EDTA and/or 0.02 % NaN3 or pass the solution through a sterile filter.
Please prevent freeze-thaw cycles.
Specific Activity
The biological activity is 16.8 U/mg, 1 unit binds 1µg biotin.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
Streptavidin Recombinant: A Versatile Tool for Biotechnology and Biomedical Research
Abstract
Streptavidin Recombinant: A Versatile Tool for Biotechnology and Biomedical Research
Introduction
Streptavidin is a widely used protein in biotechnology and biomedical research due to its exceptional binding affinity to biotin. This section introduces streptavidin and highlights its significance as a versatile tool in various applications.
Molecular Structure of Streptavidin
This section explores the molecular structure of streptavidin, including its tetrameric arrangement and the unique binding pocket for biotin. The structural features of streptavidin are crucial for understanding its robust binding properties and engineering modifications.
Binding Mechanism of Streptavidin and Biotin
The interaction between streptavidin and biotin is one of the strongest noncovalent interactions known in nature. This section explains the binding mechanism in detail, emphasizing the tight and specific association between streptavidin and biotin molecules.
Applications of Streptavidin in Biotechnology
Streptavidin's high affinity for biotin makes it an invaluable tool in various biotechnological applications. This section discusses the utilization of streptavidin in techniques such as affinity chromatography, protein purification, immunoassays, and biotinylation strategies.
Streptavidin-Based Detection Systems
Streptavidin is widely employed in detection systems, including enzyme-linked immunosorbent assays (ELISAs), Western blotting, and immunohistochemistry. This section explores the use of streptavidin-conjugated enzymes or fluorophores for sensitive and specific detection of target molecules.
Streptavidin in Bioimaging and Nanotechnology
Streptavidin's exceptional binding affinity has paved the way for its applications in bioimaging and nanotechnology. This section discusses the use of streptavidin in fluorescence imaging, nanoparticle conjugation, and the development of biosensors.
Streptavidin as a Tool for Drug Delivery
Streptavidin can be harnessed for targeted drug delivery strategies. This section explores the conjugation of streptavidin with therapeutic agents and the use of biotinylated targeting ligands for site-specific drug delivery.
Conclusion
Streptavidin recombinant is a versatile tool that plays a vital role in biotechnology and biomedical research. Its strong binding affinity for biotin enables a wide range of applications, from protein purification to bioimaging and drug delivery