prospec

Streptavidin, His

  • Name
  • Description
  • Pricings
  • Quantity
  • Streptavidin, His
  • Streptavidin Recombinant, His Tag
  • Shipped with Ice Packs

Catalogue number

PRO-621

Introduction

Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is widely used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14). The complexes are also extremely stable over a wide range of temperature and pH.

Description

Recombinant Streptomyces Avidinii Streptavidin produced in E.Coli is a single, non-glycosylated polypeptide chain (25-183) containing a total of 167 amino acids and having a molecular mass of 17kDa. The Streptavidin protein is fused to an 8 aa N-terminal His-Tag and purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The Streptavidin protein solution (1mg/ml) contains 20mM Tris-HCl pH7.5.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Amino acid sequence

MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ.

Purity

Greater than 95.0% as determined by analysis by SDS-PAGE.

References

Title:Laterally mobile, functionalized self-assembled monolayers at the fluorous-aqueous
interface in a plug based microfluidic system: characterization and testing with
membrane protein crystallization.
Publication:Department of Chemistry, The University of Chicago,929 East 57th Street, Chicago, Illinois 60637
E-mail: r-ismagilov@uchicago.edu
Link:http://ismagilovlab.caltech.edu/publications/
Ismagilov_JACS_2009_Mobile_Functional_SAMs_JEK_Supp_Info.pdf

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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