prospec

SHH Human, HEK

  • Name
  • Description
  • Pricings
  • Quantity
  • SHH Human, HEK
  • Sonic HedgeHog Human Recombinant, HEK
  • Shipped at Room temp.

Catalogue number

CYT-1079

Synonyms

SHH, HHG-1, HHG1, Sonic hedgehog protein, TPT, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5.

Introduction

Recombinant Human Sonic Hedgehog is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. 3 mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Human Sonic Hedgehog is 99% homologous to the mouse gene. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog exhibits both floor plate- and motor neuron-inducing activity. Mutations in a long-range Sonic HedgeHog enhancer located in an intron of the limb region 1 gene result in preaxial polydactyly.

Description

SHH Human Recombinant produced in HEK cells is a non-glycosylated homodimer, having a total molecular weight of 20-24kDa.

The SHH is purified by proprietary chromatographic techniques.

Source

HEK.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

SHH was lyophilized from 10mM Tris pH 7.4, 100mM NaCl and 1% CHAPS.

Solubility

It is recommended to reconstitute the lyophilized SHH in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Stability

Lyophilized SHH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SHH should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity

Greater than 90.0% as determined by analysis by SDS-PAGE.

Biological Activity

The specific activity was determined by the ability to induce alkaline phosphatase production in C3H10T1/2 mouse embryonic fibroblast cells. The EC50 is< 350ng/ml.

Usage

ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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