Inorganic pyrophosphatase, PPA.
PPA Yeast Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 286 amino acids and having a molecular mass of 35kDa.
Inorganic Pyrophosphatase is purified by proprietary chromatographic techniques.
Inorganic pyrophosphatase protein solution (100U/ml) containing 20mM Tris-HCl (25℃, pH 8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT and 50% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Do not store at -70C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Greater than 98.0% as determined by SDS-PAGE.
Under standard conditions, 1U is defined as the amount of enzyme required to catalyze the hydrolysis of pyrophosphate (PPi)/min. to produce 1μmol of orthophosphate (Pi).
Optimal reaction temp. is 25℃ , activity at 16 ~ 37℃. Cofactor: Mg+2