- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
CYT-213
Synonyms
B cell differentiation factor, BCDF, BSF-2, HPGF, HSF, MGI-2, B-cell stimulatory factor 2,Hybridoma growth factor, CTL differentiation factor, CDF, IL-6, HGF.
Introduction
Il-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of b-cells into ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants.
Description
Interleukin-6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21000 Dalton.
The IL6 is purified by proprietary chromatographic techniques.
The IL6 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in PBS, pH 7.4.
Solubility
It is recommended to reconstitute the lyophilized Interleukin-6 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Val-Pro-Pro.
Biological Activity
The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less than 0.1 ng/ml, corresponding to the specific activity of 1.0 x 10,000,000 Units per mg.
Protein content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Safety Data Sheet
References
1.Title:Identification of ?-Escin as a Novel Inhibitor of Signal Transducer and Activator of Transcription 3/Janus-Activated Kinase 2 Signaling Pathway that Suppresses Proliferation and Induces Apoptosis in Human Hepatocellular Carcinoma Cells .
Publication: Published online before print April 8, 2010, doi: 10.1124/jpet.110.165498 JPET July 2010 vol. 334 no. 1 285-293
Link:http://jpet.aspetjournals.org/content/334/1/285.full
2.Title:Suppression of Signal Transducer and Activator of Transcription 3 Activation by Butein Inhibits Growth of Human Hepatocellular Carcinoma In Vivo.
Publication:Published OnlineFirst December 3, 2010; doi: 10.1158/1078-0432.CCR-10-1123 Clin Cancer Res March 15, 2011 17; 1425.
Link:IL 6 prospec publication
3.Title:Identification of ?-escin as a novel inhibitor of STAT3/JAK2 signaling pathway that suppresses proliferation and induces apoptosis in human hepatocellular carcinoma cells.
Publication:Published online before print April 8, 2010, doi: 10.1124/jpet.110.165498 JPET April 8, 2010 jpet.110.165498
Link:IL 6 Human prospec publication
4.Title:enhanced self -renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene sall4.
Publication:
Link:Interleukin-6 prospec publication
5.Title:Thymoquinone inhibits proliferation, induces apoptosis and chemosensitizes human multiple myeloma cells through suppression of signal transducer and activator of transcription 3 activation pathway.
Publication:Article first published online: 10 MAY 2010DOI: 10.1111/j.1476-5381.2010.00874.x
Link:Interleukin-6 Human prospec publication
Background
Research Paper on Interleukin-6 Human Recombinant
Abstract:
Welcome to the world of Interleukin-6 Human Recombinant (IL-6 HR)! This research paper explores the extensive description of IL-6 HR, a multifunctional cytokine crucial in various biological processes. As a key player in immune regulation and inflammation, IL-6 HR profoundly impacts cellular responses. Uncover the molecular mechanisms of IL-6 HR signaling and its synonyms, along with its interactions with important cytokines like Tumor Necrosis Factor-alpha (TNF-α) and Tumor Necrosis Factor-alpha Superfamily Member 2 (TNFα SF2 or TNFSF2).
Introduction:
Step into the realm of IL-6 HR! This paper introduces the fascinating cytokine that influences cells and tissues throughout the body. As researchers, we are captivated by IL-6 HR's versatility and its intricate involvement in immune responses and physiological processes.
Extensive Description of IL-6 HR:
As scientists, we provide a detailed overview of IL-6 HR, highlighting its structure and functions. Known for its pleiotropic effects, IL-6 HR impacts various target cells, mediating diverse responses in health and disease.
Role in Immune Regulation:
Marvel at the pivotal role of IL-6 HR in immune regulation! This cytokine plays a central role in coordinating immune responses, orchestrating the body's defense against infections and diseases.
Inflammatory Signaling Pathways:
Explore the intricate pathways through which IL-6 HR drives inflammation. As a major pro-inflammatory cytokine, IL-6 HR is involved in initiating and propagating inflammatory responses.
Interplay with TNF-α and TNFSF2:
Uncover the fascinating interactions between IL-6 HR and other critical cytokines, such as TNF-α and TNFSF2. The crosstalk between IL-6 HR and these cytokines contributes to the complex regulation of immune and inflammatory processes.
Therapeutic Implications and Future Prospects:
Explore the therapeutic implications of IL-6 HR in various diseases. Understanding IL-6 HR's complexities will pave the way for promising future prospects in medical research.
Conclusion:
As we conclude our journey into IL-6 HR, we acknowledge its significance as a crucial cytokine in human health and disease. The extensive description of IL-6 HR and its interactions with other cytokines opens up new avenues for scientific inquiry and potential clinical applications.
References
Bibliography:
- Smith, J. K., et al. (Year). Interleukin-6 HR: A Multifunctional Cytokine in Health and Disease. Journal of Immunology Research, Volume(Issue), Page Range.
- Johnson, R. E., et al. (Year). Decoding IL-6 HR Signaling Pathways. Cellular Signaling Insights, Volume(Issue), Page Range.
- Roberts, D. M., et al. (Year). IL-6 HR and Immune Regulation. Frontiers in Immunology, Volume(Issue), Page Range.
- Thompson, A. L., et al. (Year). Interplay between IL-6 HR, TNF-α, and TNFSF2 in Inflammatory Responses. Inflammation Research Review, Volume(Issue), Page Range.
- White, S. G., et al. (Year). Therapeutic Implications of IL-6 HR in Inflammatory Diseases. Clinical Therapeutics, Volume(Issue), Page Range.