Epidermal Growth Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6.2kDa. The EGF is purified by proprietary chromatographic techniques.
EGF was lyophilized from a concentrated (1mg/ml) solution containing PBS pH-7.4.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Greater than 98.0% as determined by SDS-PAGE.
Amino acid sequence
The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0x107 IU/mg.
1.Title:Activation of the Hedgehog pathway in pilocytic astrocytomas.
Publication:Neuro Oncol (2010) 12 (8): 790-798. doi: 10.1093/neuonc/noq026 First published online: March 11, 2010
Link:EGF prospec publication
2.Title:Evaluation of bioactivity and effect of polymeric stabilizers during heat treatment for the unfolded fraction of human epidermal growth factor.
Publication:SEN'I GAKKAISHI vol.67,nov.8(2011)
Link:EGF Human prospec publication
3.Title:GPR56 is essential for testis development and male fertility in mice.
Publication:Article first published online: 27 OCT 2010 DOI: 10.1002/dvdy.22468
Link:Epidermal Growth Factor prospec publication
4.Title:Changing the Receptor Specificity of Anthrax Toxin.
Publication:doi: 10.1128/?mBio.00088-12 1 May 2012 mBio vol. 3 no. 3 e00088-12
Link:Epidermal Growth Factor Human prospec publication
5.Title:Characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins.
Publication:Kallio H, Tolvanen M, J?nis J, Pan P-w, Laurila E, et al. (2011) Characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins. PLoS ONE 6(11): e27152. doi:10.1371/journal.pone.0027152
Link:EGF Protein prospec publication
Publication: Blood 116.5 (2010): 759-766.
Link: Epidermal Growth Factor Protein prospec publication
Safety Data Sheet
Epidermal Growth Factor (EGF) Human Recombinant: Unraveling its Role in Cell Growth and Tissue Regeneration
Abstract: Epidermal Growth Factor (EGF) Human Recombinant, also known as Urogastrone (URG), is a potent growth factor that regulates cell growth, proliferation, and tissue regeneration. This research paper aims to provide a comprehensive analysis of EGF, including its molecular characteristics, signaling pathways, physiological functions, and clinical implications. The paper explores the significance of EGF as a key regulator in various cellular processes and its potential applications in tissue engineering and regenerative medicine.
- Introduction Epidermal Growth Factor (EGF) is a growth factor with diverse roles in cellular processes and tissue homeostasis. This section introduces EGF and provides an overview of its synonyms, including Urogastrone (URG) and EGF, highlighting their relevance in scientific literature.
- Molecular Characteristics of EGF EGF is a small, single-chain polypeptide consisting of 53 amino acids. This section discusses the structural characteristics of EGF, including its primary sequence, three-dimensional structure, and post-translational modifications. The importance of these molecular features in EGF's biological activity is emphasized.
- Signaling Pathways of EGF EGF exerts its effects through the binding and activation of the EGF receptor (EGFR). This section delves into the intracellular signaling pathways activated by EGF, such as the MAPK/ERK pathway and the PI3K/Akt pathway. The downstream effectors and their roles in mediating cellular responses to EGF stimulation are explored.
- Physiological Functions of EGF EGF plays a crucial role in various physiological processes, including embryonic development, tissue regeneration, wound healing, and epithelial cell growth. This section provides an in-depth analysis of EGF's physiological functions, highlighting its involvement in cell proliferation, differentiation, migration, and survival. The influence of EGF on epithelial tissues, such as the skin and gastrointestinal tract, is also discussed.
- Clinical Implications of EGF EGF has shown promising therapeutic potential in wound healing, skin regeneration, and the treatment of certain diseases. This section discusses the clinical implications of EGF, including its use in tissue engineering, wound dressings, and the development of EGF-based therapies. The challenges and future prospects associated with harnessing EGF for clinical applications are addressed.
- Conclusion Epidermal Growth Factor (EGF), also known as Urogastrone (URG) or EGF, is a potent growth factor that plays a critical role in cell growth, tissue regeneration, and epithelial homeostasis. This research paper has provided a comprehensive analysis of EGF, highlighting its molecular characteristics, signaling pathways, physiological functions, and clinical implications. Further research on EGF will deepen our understanding of its complex mechanisms and open avenues for innovative therapeutic strategies.