prospec

EGF Mouse, Biotin

  • Name
  • Description
  • Pricings
  • Quantity
  • EGF Mouse, Biotin
  • Epidermal Growth Factor Mouse Recombinant, Biotin
  • Shipped with Ice Packs

Catalogue number

CYT-841

Synonyms

Urogastrone, URG, EGF.

Introduction

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.

Description

EGF Mouse Recombinant, Biotin produced in E.Coli is a non-glycosylated polypeptide chain containing 61 amino acids and having a total molecular mass of 7.0kDa. This version of EGF has a N terminal leader sequence hosting a biotin conjugation. There are 0.5 biotins for each EGF protein. 

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

The protein (0.5mg/ml) solution contains sterile PBS.

Stability

Should be stored at 4°C.
Please do not freeze.

Purity

Greater than 95.0% as determined by analysis by SDS-PAGE.

Amino acid sequence

MKKIDDDKNS YPGCPSSYDG YCLNGGVCMH IESLDSYTCN CVIGYSGDRC QTRDLEWWEL R. 

Biological Activity

The ED50 as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells is 0.14-0.2ng/ml, corresponding to a specific activity of 7.1x106units/mg.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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