prospec

DKK1 Human, HEK

  • Name
  • Description
  • Pricings
  • Quantity
  • DKK1 Human, HEK
  • Dickkopf-Related Protein 1 Human Recombinant, HEK
  • Shipped at Room temp.

Catalogue number

PRO-2405

Synonyms

Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK, DKK1, dickkopf WNT signaling pathway inhibitor 1, Dickkopf 1 Homolog, Dickkopf Related Protein-1, Dickkopf-1 Like, Dickkopf-Like Protein 1, Dickkopf (Xenopus Laevis) Homolog 1, DKK-1.

Introduction

Dickkopf-related protein 1 (DKK1) antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by creating a ternary complex with the transmembrane protein KREMEN which promotes internalization of LRP5/6. DKKs have a significant role in vertebrate development, where they locally inhibit Wnt controlled processes for instance antero-posterior axial patterning, limb development, somitogenesis and eye formation. Furthermore, Dkks are involved in bone formation and bone disease, cancer and Alzheimer disease in Adults.

Description

DKK1 Human Recombinant produced in HEK cells is a glycoprotein containing 235 amino acid residues.
DKK1 Human which has a molecular weight of 25.8kDa is purified by proprietary chromatographic techniques.

Source

HEK.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The DKK1 protein was lyophilized from solution containing 1x PBS, pH 7.4 .

Solubility

It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. 

Purity

Greater than 97.0% as determined by analysis by SDS-PAGE and HPLC analysis.

Biological Activity

The specific activity was determined by its ability to inhibit the proliferation
of HCT116 colorectal carcinoma cells. Approximately 40% growth inhibition was
achieved at a DKK-1 concentration of 200ng/ml.

Amino acid sequence

TLNSVLNSN IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC
GTDEYCASPT RGGDAGVQIC LACRKRRKRC MRHAMCCPGN YCKNGICVSS DQNHFRGEIE
ETITESFGND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK
PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSRLH TCQRH.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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