DKK1 Human Recombinant produced in HEK cells is a glycoprotein containing 235 amino acid residues.
DKK1 Human which has a molecular weight of 25.8kDa is purified by proprietary chromatographic techniques.
Sterile Filtered White lyophilized (freeze-dried) powder.
The DKK1 protein was lyophilized from solution containing 1x PBS, pH 7.4 .
It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Greater than 97.0% as determined by analysis by SDS-PAGE and HPLC analysis.
The specific activity was determined by its ability to inhibit the proliferation
of HCT116 colorectal carcinoma cells. Approximately 40% growth inhibition was
achieved at a DKK-1 concentration of 200ng/ml.
Amino acid sequence
TLNSVLNSN IKNLPPPLGG AAGHPGSAVS AAPGILYPGG NKYQTIDNYQ PYPCAEDEEC
GTDEYCASPT RGGDAGVQIC LACRKRRKRC MRHAMCCPGN YCKNGICVSS DQNHFRGEIE
ETITESFGND HSTLDGYSRR TTLSSKMYHT KGQEGSVCLR SSDCASGLCC ARHFWSKICK
PVLKEGQVCT KHRRKGSHGL EIFQRCYCGE GLSCRIQKDH HQASNSSRLH TCQRH.