CKMM1 Human

CKMM1 Human

Creatine Kinases

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  • Name :

    CKMM1 Human

    Description:

    Creatine Kinase Muscle Type-1 Human Recombinant

    Creatine kinase M-type, EC 2.7.3.2, Creatine kinase M chain, M-CK, CKM, CKMM, CKMMITI.

    Product # :

    CKI-271

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    Shipped at Room temp

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  • description
  • source
  • formulation
  • purity
  • biological activity
  • More Info

Description

CKMM Type-1 Human Recombinant produced in E.Coli is a noncovalently linked homodimer, consisting of 2 x 380 amino acid polypeptide chains, having a total Mw of 86kDa.

Source

E.Coli

Formulation

CKMT1 contains 20mM Tris, 150mM NaCl, 1mM TCEP, pH-8

Purity

Greater than 95.0% as determined by SDS-PAGE

Biological Activity

The enzymatic activity was measured by its ability to phosphorylate creatine at room temperature. The specific activity is > 1,900 pmol/min/μg..

More Info

  • Introduction

    Creatine Kinase MM is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

  • Synonyms

    Creatine kinase M-type, EC 2.7.3.2, Creatine kinase M chain, M-CK, CKM, CKMM, CKMMITI.

  • Physical Appearance

    Sterile Filtered colourless liquid formulation.

  • Stability

    Lyophilized CKMM type-1 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CKMM should be stored at 4°C between 2-7 days and for future use below -18°C.
    Please prevent freeze-thaw cycles.

  • Solubility

    It is recommended to reconstitute the lyophilized CKMM type-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

  • Amino Acid Sequence

    MPFGNTHNKF KLNYKPEEEY PDLSKHNNHM AKVLTLELYK KLRDKETPSG FTVDDVIQTG VDNPGHPFIM TVGCVAGDEE SYEVFKELFD PIISDRHGGY KPTDKHKTDL NHENLKGGDD LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT EKEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKSFLVWVN EEDHLRVISM EKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNQHLGYV LTCPSNLGTG LRGGVHVKLA HLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDVSNAD RLGSSEVEQV QLVVDGVKLM VEMEKKLEKG QSIDDMIPAQ

  • Background

    What is the molecular weight / Mw of CKMM type-1 Protein?
    CKMM type-1 Protein is a non covalently homodimer having a total Mw of 86kda

    What is the source or expression system of CKMM type-1 Protein?
    Escherichia Coli.

    What is the Purity of CKMM type-1 Protein?
    CKMM Protein is >95% pure as determined by SDS-PAGE.

    What is the Biological Activity of CKMM type-1 Protein?
    The enzymatic activity was determined by its ability to phosphorylate creatine at room temperature. The specific activity is > 1,900 pmol/min/μg.

    What is the amino acid sequence of CKMM type-1 Protein?
    MPFGNTHNKF KLNYKPEEEY PDLSKHNNHM AKVLTLELYK KLRDKETPSG FTVDDVIQTG VDNPGHPFIM TVGCVAGDEE SYEVFKELFD PIISDRHGGY KPTDKHKTDL NHENLKGGDD LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT EKEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKSFLVWVN EEDHLRVISM EKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNQHLGYV LTCPSNLGTG LRGGVHVKLA HLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDVSNAD RLGSSEVEQV QLVVDGVKLM VEMEKKLEKG QSIDDMIPAQ

    What applications can CKMM type-1 Protein be used in?
    CKMM Protein can probably be used in western blot, ELISA and Lateral Flow.

    What is the endotoxin level for CKMM type-1 Protein?
    The endotoxin level is minimal, CKMM type-1 Protein was purified using conventional chromatography techniques.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Ckmmiti Human

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