prospec

Carboxypeptidase B Rat

  • Name
  • Description
  • Pricings
  • Quantity
  • Carboxypeptidase B Rat
  • Carboxypeptidase-B Rat Recombinant
  • Shipped at Room temp.

Catalogue number

ENZ-475

Synonyms

Carboxypeptidase B, Cpb1, Cpb.

Introduction

Carboxypeptidase B (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 34.5 kDa, optimun pH-7.9, and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA, 1,10-phenanthroline.

Description

Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a Mw of 31kDa is purified by standard chromatography techniques.
Recombinant Rat Carboxypeptidase-B is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Carboxypeptidase-B is free from protease inhibitors such as PMSF and EDTA.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered lyophilized powder.

Formulation

The protein was lyophilized with 100mM NaCl, mannitol and 20mM Tris pH-7.5.

Solubility

It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile 18MΩ-cm H2O or 25mM Tris-HCl pH 7.65 not less than 100µg/ml , which can then be further diluted to other aqueous solutions.

Stability

Store the lyophilized Carboxypeptidase-B at 4°C. Upon reconstitute the protein should be stored at 4°C for 2 weeks and for future use below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 90% as determined by SDS-PAGE.

Unit Definition

One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH-7.65.

Biological Activity

170 units/mg protein.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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