- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
CYT-579
Synonyms
Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.
Introduction
NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.
Description
Nerve Growth Factor-beta Human Recombinant produced in E.Coli is a non-covalently disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 identical 121 amino acids with a molecular weight of two 13.6 kDa polypeptide monomers.
The NGF-b is purified by proprietary chromatographic techniques.
The NGF-b is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The beta-NGF protein was lyophilized from a 0.2µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA).
Solubility
It is recommended to reconstitute the lyophilized NGF-b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized Beta-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NGF-Beta should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MSSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA.
Biological Activity
The ED50, calculated by its ability to stimulate proliferation of TF-1 cells and is typically 1.31 ng/ml, corresponding to a specific activity of 7.6x105units/mg.
References
Title: u-Opioid receptor activation in live cells
Publication: Journal 22.10 (2008): 3537-3548.
Link: b NGF prospec publication
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Background
Beta Nerve Growth Factor Human Recombinant: Unlocking its Therapeutic Potential in Neuroregeneration
Abstract:
Beta Nerve Growth Factor (β-NGF) human recombinant is a critical neurotrophic factor that plays a pivotal role in promoting neuronal survival, growth, and differentiation. This research paper aims to provide a comprehensive analysis of β-NGF, including its characteristics, signaling pathways, and potential therapeutic applications in neuroregeneration. Additionally, innovative methodologies for the production and optimization of β-NGF human recombinant are proposed, highlighting its promising prospects in the field of neuroscience and regenerative medicine.
Introduction:
Neuroregeneration is a complex process that requires the support of various factors to promote the survival and growth of neurons. β-NGF, a prominent member of the neurotrophin family, acts as a crucial mediator in neuronal development and regeneration. This paper explores the unique features of β-NGF and presents novel approaches for the production and optimization of β-NGF human recombinant, aiming to unlock its therapeutic potential in diverse neuroregenerative contexts.
Characteristics and Signaling Pathways:
β-NGF is a secreted protein that binds to specific cell surface receptors, initiating intracellular signaling pathways crucial for neuronal function and maintenance. The activation of TrkA receptors and downstream signaling cascades, including the MAPK/ERK and PI3K/Akt pathways, promotes neuronal survival, axonal growth, and synaptic plasticity. The intricate interplay between β-NGF and its receptors regulates the development, maintenance, and repair of the nervous system.
Production of β-NGF Human Recombinant:
Efficient production methodologies are essential for harnessing the therapeutic potential of β-NGF human recombinant. Various recombinant protein expression systems, such as bacterial expression systems or mammalian cell culture systems, have been employed to produce functional β-NGF. Optimization strategies, including codon optimization, secretion signal engineering, and protein purification techniques, have been implemented to enhance the yield, stability, and bioactivity of β-NGF recombinant protein.
Potential Therapeutic Applications:
β-NGF human recombinant holds immense promise in the field of neuroregeneration and regenerative medicine. Its ability to promote neuronal survival, axonal growth, and synaptic plasticity makes it a potential candidate for the treatment of neurodegenerative disorders, peripheral nerve injuries, and central nervous system injuries. Furthermore, the neuroprotective properties of β-NGF suggest its broader therapeutic applications in neurological disorders and brain injuries.
Conclusion:
β-NGF human recombinant emerges as a critical factor in neuroregeneration, offering significant potential for neuronal repair and restoration. Optimizing production methodologies and further elucidating its signaling mechanisms will undoubtedly enhance its therapeutic applications. Given its role in promoting neuronal survival, growth, and plasticity, β-NGF human recombinant represents a valuable tool for advancing neuroregeneration and addressing the unmet clinical needs in neurology and regenerative medicine.
References
Bibliography:
- Chao MV. Neurotrophins and their receptors: A convergence point for many signalling pathways. Nat Rev Neurosci. 2003;4(4):299-309.
- Crowley C, Spencer SD, Nishimura MC, et al. Mice lacking nerve growth factor display perinatal loss of sensory and sympathetic neurons yet develop basal forebrain cholinergic neurons. Cell. 1994;76(6):1001-1011.
- Levi-Montalcini R, Hamburger V. Selective growth stimulating effects of mouse sarcoma on the sensory and sympathetic nervous system of the chick embryo. J Exp Zool. 1951;116(2):321-361.
- Sofroniew MV, Howe CL, Mobley WC. Nerve growth factor signaling, neuroprotection, and neural repair. Annu Rev Neurosci. 2001;24:1217-1281.
- Teng HK, Teng KK, Lee R, et al. ProBDNF induces neuronal apoptosis via activation of a receptor complex of p75NTR and sortilin. J Neurosci. 2005;25(22):5455-5463.