The NGF-b is purified by proprietary chromatographic techniques.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Amino acid sequence
The ED50, calculated by its ability to stimulate proliferation of TF-1 cells and is typically 1.31 ng/ml, corresponding to a specific activity of 7.6x105units/mg.
Title: u-Opioid receptor activation in live cells
Publication: Journal 22.10 (2008): 3537-3548.
Link: b NGF prospec publication
Safety Data Sheet
Beta Nerve Growth Factor Human Recombinant: Unlocking its Therapeutic Potential in Neuroregeneration
Beta Nerve Growth Factor (β-NGF) human recombinant is a critical neurotrophic factor that plays a pivotal role in promoting neuronal survival, growth, and differentiation. This research paper aims to provide a comprehensive analysis of β-NGF, including its characteristics, signaling pathways, and potential therapeutic applications in neuroregeneration. Additionally, innovative methodologies for the production and optimization of β-NGF human recombinant are proposed, highlighting its promising prospects in the field of neuroscience and regenerative medicine.
Neuroregeneration is a complex process that requires the support of various factors to promote the survival and growth of neurons. β-NGF, a prominent member of the neurotrophin family, acts as a crucial mediator in neuronal development and regeneration. This paper explores the unique features of β-NGF and presents novel approaches for the production and optimization of β-NGF human recombinant, aiming to unlock its therapeutic potential in diverse neuroregenerative contexts.
Characteristics and Signaling Pathways:
β-NGF is a secreted protein that binds to specific cell surface receptors, initiating intracellular signaling pathways crucial for neuronal function and maintenance. The activation of TrkA receptors and downstream signaling cascades, including the MAPK/ERK and PI3K/Akt pathways, promotes neuronal survival, axonal growth, and synaptic plasticity. The intricate interplay between β-NGF and its receptors regulates the development, maintenance, and repair of the nervous system.
Production of β-NGF Human Recombinant:
Efficient production methodologies are essential for harnessing the therapeutic potential of β-NGF human recombinant. Various recombinant protein expression systems, such as bacterial expression systems or mammalian cell culture systems, have been employed to produce functional β-NGF. Optimization strategies, including codon optimization, secretion signal engineering, and protein purification techniques, have been implemented to enhance the yield, stability, and bioactivity of β-NGF recombinant protein.
Potential Therapeutic Applications:
β-NGF human recombinant holds immense promise in the field of neuroregeneration and regenerative medicine. Its ability to promote neuronal survival, axonal growth, and synaptic plasticity makes it a potential candidate for the treatment of neurodegenerative disorders, peripheral nerve injuries, and central nervous system injuries. Furthermore, the neuroprotective properties of β-NGF suggest its broader therapeutic applications in neurological disorders and brain injuries.
β-NGF human recombinant emerges as a critical factor in neuroregeneration, offering significant potential for neuronal repair and restoration. Optimizing production methodologies and further elucidating its signaling mechanisms will undoubtedly enhance its therapeutic applications. Given its role in promoting neuronal survival, growth, and plasticity, β-NGF human recombinant represents a valuable tool for advancing neuroregeneration and addressing the unmet clinical needs in neurology and regenerative medicine.
- Chao MV. Neurotrophins and their receptors: A convergence point for many signalling pathways. Nat Rev Neurosci. 2003;4(4):299-309.
- Crowley C, Spencer SD, Nishimura MC, et al. Mice lacking nerve growth factor display perinatal loss of sensory and sympathetic neurons yet develop basal forebrain cholinergic neurons. Cell. 1994;76(6):1001-1011.
- Levi-Montalcini R, Hamburger V. Selective growth stimulating effects of mouse sarcoma on the sensory and sympathetic nervous system of the chick embryo. J Exp Zool. 1951;116(2):321-361.
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- Teng HK, Teng KK, Lee R, et al. ProBDNF induces neuronal apoptosis via activation of a receptor complex of p75NTR and sortilin. J Neurosci. 2005;25(22):5455-5463.