prospec

Urease

  • Name
  • Description
  • Pricings
  • Quantity
  • Urease
  • Urease Recombinant
  • Shipped at Room temp.

Catalogue number

ENZ-277

Description

The mutant Urease from microorganism source, showing shifted substrate affinity to urea. It was designed wildtype coding gene from microorganism. 
The subunit structure is very similar to well known microbial urease.
Please refer to published literature such as JBC 262, 5963-67 (1987).
It is composed of multi-subunits and shows a bit complex protein structure (alpha 2 Beta 4 Gamma 4) as compared to plant urease
rUrease is genetically designed unique mutant having shifted high Km to urea, which is suited material to kinetic urea assay with wide measurable range.
The enzyme comprises of three different subunits to make complete fully active form, 60.3 kD a subunit, 11.7 kD b subunit and 11.1 kD g subunit respectively.

Source

Escherichia Coli.

Physical Appearance

Sterile Lyophilized Powder.

Formulation

Each mg of protein contains 420µg Potassium Phosphate and 30µg EDTA Na2.

Solubility

It is recommended to reconstitute the lyophilized Urease in sterile 18MΩ-cm H2O.

Stability

Urease although stable at 4°C for 3 weeks, should be stored desiccated below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Unit Definition

One Unit oxidizes one micromole of NADH per minute at 25°C, at pH 7.6.

Biological Activity

The activity was found to be 141U/mg powder.

Safety Data Sheet

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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