TROVE2 His is a recombinant protein having a molecular mass of 64,000 Dalton (pH 8.3-8.6 (depending on prediction program). TROVE2 is fused to a hexa-histidine purification tag.
TROVE2 is supplied in 20mM HEPES buffer pH-8.0, 150mM NaCl, 1mM DTT and 10% glycerol.
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checkerboard analysis of positive/negative samples); line assay and immunodot test with positive/negative samples.
Western blot with anti TROVE2 His Human autoantibody positive sample and monoclonal anti-His-tag antibody.
0.2-0.5 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
Greater than 95% as determined by SDS-PAGE.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.