Non-specific lipid-transfer protein, ltp142.
Tri a 14.0101 is a non-specific lipid transfer protein and a major wheat allergen. Those who are sensitive to this allergen may have baker’s asthma, a frequent occupational allergic disease caused mainly by inhalation of cereal flour, especially wheat flour. Tri a 14.0101 consists of 4 helical fragments and irregular C-terminal regions which are highly stable to heat treatment and proteolytic attack.
Recombinant Non-Specific Lipid-Transfer Protein Tri a 14 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 13kDa.
Tri a 14.0101 is expressed with a 6xHis tag and purified by proprietary chromatographic techniques.
Sf9 insect cells.
Sterile Filtered clear solution.
Tri a 14.0101 is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl and 20% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Greater than 80.0% as determined by SDS-PAGE.
1. Binds IgE-type human antibodies.
2. Immunodot test with positive/negative samples.
Tested by LAL (Limulus Amoebocyte Lysate) chromogenic endotoxin assay.