About Transcription Elongation Factor:
Elongation factors are a set of proteins functioning at the ribosome, throughout the process of protein synthesis, that help translational elongational f the first to the last peptide bond of a growing polypeptide. The transcription elongation factors include Transcription Elongation Factor A1 (TCEA1), Transcription Elongation Factor A3 (TCEA2), and Transcription Elongation Factor A3 (TCEA3)
Transcription Elongation Factor Function
TCEA1 primarily helps to resolve backtracked elongation complexes by making a cut in the RNAP active site and is also present in the eukaryotic transcription preinitiation complex. TCEA2 is found in the nucleus and releases RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. TCEA3 promotes muscle-specific gene expression during myogenesis
Transcription Elongation Factor Interactions
TCEA1 interacts with POLR2A (RNA Polymerase II, I And III Subunit A), POLR2E (RNA Polymerase II, I And III Subunit E), POLR2F (RNA Polymerase II, I And III Subunit F), GTF2F2 (General Transcription Factor IIF Subunit 2), and GTF2B (General Transcription Factor IIB).
TCEA2 interacts with POLR2F, POLR2H (RNA Polymerase II, I And III Subunit H), POLR2I (RNA Polymerase II, I And III Subunit I), POLR2K (RNA Polymerase II, I And III Subunit K) and GTF2B.
TCEA3 interacts with POLR2F, POLR2H, POLR2I, POLR2K, and POLR2L (RNA Polymerase II, I And III Subunit L).
Transcription Elongation Factor Structure
TCEA1 has a size of 301 amino acids and a molecular mass of 33970 DA, and a quaternary structure that interacts with EAF2 and associates with UBR5 and formats a transcription regulatory complex made of CDK9, RNAP II, UBR5, and TFIIS/TCEA1 that can stimulate target gene transcription (e.g., gamma fibrinogen/FGG) by recruiting their promoters.