Recombinant TOP1 70kDa protein is an enzyme fragment having a molecular mass of 72KDa (pH 9.4). TOP1 70kDa protein is fused to a hexa-histidine purification tag.
TOP1 70kDa is supplied in 20mM HEPES buffer pH-8.0, 500mM NaCl, and 20% glycerol.
1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checkerboard analysis of positive/negative samples) and immunodot test with positive/negative samples.
Western blot with anti TOP1 70kDa autoantibody positive sample or monoclonal anti-hexa-His-tag antibody.
0.5-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
Greater than 95% as determined by SDS-PAGE.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.