prospec

Thrombin Human, HEK

  • Name
  • Description
  • Pricings
  • Quantity
  • Thrombin Human, HEK
  • Thrombin Human Recombinant, HEK
  • Shipped with Ice Packs

Catalogue number

PRO-1422

Synonyms

Prothrombin, EC 3.4.21.5, Coagulation factor II, F2, PT, THPH1, RPRGL2.

Introduction

Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.

Description

Recombinant Human Thrombin produced in HEK cells, having a total molecular weight of 36kDa.
The Thrombin is purified by proprietary chromatographic techniques.

Source

HEK

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The Thrombin solution contains 20mM MES, pH6.0 and 500mM Choline Chloride.

Stability

Store frozen at -20°C to -80°C for long periods of time. Avoid multiple freeze-thaw cycles.

Assay Conditions

Thrombin (1nM) was assayed using SPECTROZYME TH as a substrate (20µM) in 5mM Tris-HCl (pH8.0), 0.1% PEG, 200mM NaCl at 25°C.

Biological Activity

5396 NIH Units/mg.
The activity was determined in NIH units by comparing to Sigma’s human plasma thrombin. Protein concentration was measured using E(0.1%)@280nm = 1.83.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Back to Top