Thrombin Bovine

Thrombin Bovine

  • Name
  • Description
  • Cat#
  • Pricings
  • Quantity
  • Thrombin Bovine

  • Bovine Thrombin
  • PRO-447
  • Shipped at Room temp.

Catalogue number



Coagulation Factor II (Thrombin), Prepro-Coagulation Factor II, EC, RPRGL2, THPH1, Coagulation Factor II, Prothrombin B-Chain, Serine Protease, Prothrombin, EC 3.4.21, PT, F2.


Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.


Bovine Blood.

Physical Appearance

Sterile Filtered beige lyophilized (freeze-dried) powder.


Lyophilized freeze dry powder formulated with glycine, CaCl2 and sodium chloride, PH 6.8.


It is recommended to reconstitute the lyophilized Thrombin in 0.9% NaCl.

Biological Activity

164 US unites/mg protein.


Lyophilized Thrombin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Thrmbin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Safety Data Sheet


ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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