TGFB1 Human, HEK

Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.

Transforming growth factor betas (TGFBetas) mediate many cell-cell interactions that occur during embryonic development. 3 TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains linked with the latent portion of the molecule.

TGFB1 Human Recombinant produced in 293 cells is a glycosylated homodimeric polypeptide chain having an apparent molecular mass of 25kDa.

The TGFB1 is purified by proprietary chromatographic techniques.

HEK293 Cells.

Lyophilized from a sterile filtered solution containing 50mM NaOAc pH-4.5

It is recommended to reconstitute the lyophilized TGFB1 in sterile 5mM HCL at a concentration of 0.1 mg/ml, which can then be further diluted to other aqueous solutions.

Lyophilized TGFB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB1 Human should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Greater than 95.0% as determined by SDS-PAGE.

The ED₅₀ as determined by the dose-dependent inhibition of IL-4-induced proliferation of HT-2 cells is 0.15ng/ml, corresponding to a specific activity of 6.6x10⁶units/mg.

SDS

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.