prospec

Streptolysin-O, His

  • Name
  • Description
  • Pricings
  • Quantity
  • Streptolysin-O, His
  • Streptococcus Pyogenes Streptolysin-O Recombinant, His Tag
  • Shipped with Ice Packs

Catalogue number

PRO-2574

Synonyms

Streptolysin O, Thiol-activated cytolysin, slo.

Introduction

Streptolysin-O is a sulfhydryl-activated toxin which causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the Streptolysin-O protein undergoes a major conformation change, leading to its insertion in the host membrane and creation of an oligomeric pore complex. Cholesterol may be needed for binding to host membranes, membrane insertion and pore formation. Streptolysin-O can be reversibly inactivated by oxidation.

Description

Recombinant Streptolysin-O produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 61kDa.

Streptolysin-O is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

Streptolysin-O is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.

Store, frozen at -20°C for longer periods of time.

Avoid multiple freeze-thaw cycles.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Immunological Activity

1. Binds IgG- and IgM-type human antibodies.
2. Immunodot test with positive/negative samples.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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