Recombinant Streptolysin-O produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 61kDa.
Streptolysin-O is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Sterile Filtered clear solution.
Streptolysin-O is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Greater than 80.0% as determined by SDS-PAGE.
2. Immunodot test with positive/negative samples.