- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
PRO-2574
Synonyms
Streptolysin O, Thiol-activated cytolysin, slo.
Introduction
Streptolysin-O is a sulfhydryl-activated toxin which causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the Streptolysin-O protein undergoes a major conformation change, leading to its insertion in the host membrane and creation of an oligomeric pore complex. Cholesterol may be needed for binding to host membranes, membrane insertion and pore formation. Streptolysin-O can be reversibly inactivated by oxidation.
Description
Recombinant Streptolysin-O produced in E.coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 61kDa.
Streptolysin-O is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
Streptolysin-O is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Immunological Activity
1. Binds IgG- and IgM-type human antibodies.
2. Immunodot test with positive/negative samples.
2. Immunodot test with positive/negative samples.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.