Single-Stranded DNA Binding Protein

Single-Stranded DNA Binding Protein

Single-Stranded DNA Binding Protein

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  • View Data Sheet

    Description:

    Single-Stranded DNA Binding Protein 1 Human Recombinant, His Tag

    Mt-SSB, mtSSB, SOSS-B1, SSBP, PWP1-interacting protein 17, Single-stranded DNA-binding protein, mitochondrial.

    Product # :

    PRO-1408

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    • description
    • source
    • formulation
    • purity
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    Description

    SSBP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 155 amino acids (17-148 a.a.) and having a molecular mass of 17kDa.SSBP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    SSBP1 protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer, (pH 8.0), 0.15M NaCl, 30% glycerol and 1mM DTT.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      Single-Stranded DNA Binding Protein 1 (SSBP) participates in mitochondrial biogenesis. SSBP binds preferentially and cooperatively to ss-DNA. The SSBP protein is involved in mitochondrial DNA replication and associates with mitochondrial DNA.

    • Synonyms

      Mt-SSB, mtSSB, SOSS-B1, SSBP, PWP1-interacting protein 17, Single-stranded DNA-binding protein, mitochondrial.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSESETTTS LVLERSLNRV HLLGRVGQDP VLRQVEGKNP VTIFSLATNE MWRSGDSEVY QLGDVSQKTT WHRISVFRPG LRDVAYQYVK KGSRIYLEGK IDYGEYMDKN NVRRQATTII ADNIIFLSDQ TKEKE.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Ssbp1 Human His
  • View Data Sheet

    Name :

    SSBP1

    Description:

    Single-Stranded DNA Binding Protein 1 Sulfolobus solfataricus Recombinant

    Mt-SSB, mtSSB, SOSS-B1, SSBP, PWP1-interacting protein 17, Single-stranded DNA-binding protein, mitochondrial.

    Product # :

    PRO-359

    Price :

    Quantity :

    Shipping Method :

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    Shipped with Ice Packs

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    More Info

    • description
    • source
    • formulation
    • purity
    • More Info

    Description

    Recombinant Sulfolobus solfataricus Single-Stranded DNA Binding Protein 1 produced in E.coli cells is a non-glycosylated, homodimeric protein containing 148 amino acids and having a molecular mass of 16.1kDa. The SSBP1 is purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    SSBP1 is a 0.2µm filtered concentrated solution which contains 20mM Tris, pH 7.4, 200mM NaCl, 1mM EDTA, 0.5mM DTT, and 50% Glycerol.

    Purity

    Greater than 95.0% as determined by:
    (a) Analysis by RP-HPLC.
    (b) Analysis by SDS-PAGE.

    More Info

    • Introduction

      Single-Stranded DNA Binding Protein 1 (SSBP) participates in mitochondrial biogenesis. SSBP binds preferentially and cooperatively to ss-DNA. The SSBP protein is involved in mitochondrial DNA replication and associates with mitochondrial DNA.

    • Synonyms

      Mt-SSB, mtSSB, SOSS-B1, SSBP, PWP1-interacting protein 17, Single-stranded DNA-binding protein, mitochondrial.

    • Physical Appearance

      Sterile Filtered clear solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MEEKVGNLKP NMESVNVTVR VLEASEARQI QTKNGVRTIS EAIVGDETGR VKLTLWGKHA GSIKEGQVVK IENAWTTAFK GQVQLNAGSK TKIAEASEDG FPESSQIPEN TPTAPQQMRG GGRGFRGGGR RYGRRGGRRQ ENEEGEEE

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Ssbp1

About SSBP1 / Single-Stranded DNA Binding Protein:

DNA is present first and foremost as a duplex to stabilize and protect our genome. Single-Stranded DNA Binding Protein (SSBP) plays a role in DNA replication, recombination, and repair. Due to many cellular processes, like replication and transcription, single-stranded DNA (ssDNA) is exposed.

SSBP1 Mechanism
Single-Stranded Binding Proteins stabilize the unwound DNA. SSB molecules bind to the newly separated individual DNA strands during DNA replication. What this does is keep the strands separated by holding them in place. The purpose is so that each strand can serve as a template for new DNA synthesis.

Single-Stranded DNA Binding Protein Interactions
Single-stranded DNA-binding proteins bind and protect ssDNA intermediates. These are formed during replication, recombination, and repair reactions. These proteins also interact with several different genome maintenance proteins which then stimulate their enzymatic activities, as well as control and make their proper cellular localization possible.

SSBP1 Function
They’re essential proteins found in all domains of life. Single strand DNA binding proteins are vital during DNA synthesis. It’s because they work to prevent the coiling of the separated DNA strands. The distinguishing functional unit of the SSBs is the oligonucleotide/oligosaccharide-binding (OB)-fold. This is a protein domain that makes the binding to ssDNA possible, as well as various protein-protein interactions.

Single-Stranded DNA Binding Protein Structure
Single-strand DNA binding protein is a protein found in Escherichia coli (E. coli) bacteria. SSB protein domains in bacteria are important in their function of maintaining DNA metabolism. It has a structure of three beta-strands to a single six-stranded beta-sheet which forms a protein dimer. Single-stranded DNA can fold up into different shapes since it's not as stiff as double-stranded DNA.