prospec

Protein A, 41kDa

  • Name
  • Description
  • Pricings
  • Quantity
  • Protein A, 41kDa
  • Staphylococcal Protein A 41kDa Recombinant
  • Shipped at Room temp.

Catalogue number

PRO-774

Synonyms

Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SPA.

Introduction

Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. It couples to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. Recombinant Protein A was developed to increase the specificity of the molecule for IgG and is widely used both in research and bioprocessing. The recombinant protein A is produced by expressing a modified protein A gene in E.coli. A specific purification process with strict quality control was taken to get the recombinant protein A with the purity of more than 98% , no human IgG affinity step is used during validated fermentation and purification and devoid of bacterial contaminant found normally in native Protein A. (Free of Stap

Description

Recombinant Staphylococcal Protein A produced in E.Coli is a non-glycosylated, Polypeptide chain contaning the amino acid sequence of Staphylococcal Protein A IgG binding domains having a molecular mass of 41 kDa. The recombinant Protein A contains 6 IgG-binding regions of protein A. Cell wall binding region, albumin binding region and other non-specific binding regions that have been eliminated from the recombinant Protein-A to ensure the maximum specific IgG binding. Recombinant Staphylococcal Protein A is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution containing no additives.

Stability

Protein-A should be stored at all times at 4°C.

Purity

Greater than 98.0% as determined by SDS-PAGE & RP-HPLC.

Biological Activity

7mg human IgG/mg protein-A.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Back to Top