Calcitonin, Preprocalcitonin, Calca.
Procalcitonin is a peptide hormone mainly produced by the C cells of the thyroid and certain endocrine cells of the lung. Under normal expression conditions, procalcitonin is immediately cleaved into three specific fragments, an N terminal residue, calcitonin and katacalcin. Levels of unprocessed procalcitonin rise significantly after bacterial infection, trauma or shock.
Procalcitonin Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Thr26-Asn141) containing 126 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 14.1kDa.
Filtered White lyophilized (freeze-dried) powder.
Procalcitonin was filtered (0.4µm) and lyophilized in 20mM TRIS and 50mM NaCl, pH 8.0.
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Procalcitonin is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Amino acid sequence
MKHHHHHHASTPLRSALETL PDPGPLSEKE GRLLLAALVK AYVQRKTNEL EQEQEQETEG SSLDSSRAKR CSNLSTCVLS AYWRNLNNFH RFSGMGFGPE TPGKKSDIAS SLERDLFPRG MPQDAN.
Greater than 95.0% as determined by SDS-PAGE.
Safety Data Sheet
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.