PODXL Human (335-387)

The PODXL Human is created as a recombinant protein with a 4kda N-terminal fusion of His Tag. The PODXL His-Tagged Fusion Protein, produced in E. coli, is a 11kDa protein containing 53 amino acid residues of the PODXL Human, 335-387 amino acids.

Escherichia Coli.

Filtered White lyophilized (freeze-dried) powder.

Each mg was lyophilized with 1xPBS, 0.4% SDS and 4mM DTT.

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.

Store lyophilized PODXLat -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

AGGASDEKLI SLICRAVKAT FNPAQDKCGI RLASVPGSQT VVVKEITIHS. 

Greater than 90% as determined by SDS-PAGE.

SDS

Podocalyxin-Like also known as PODXL is a glycosylated transmembrane sialoprotein in the CD34 and endoglycan family. The PODXL protein takes part in the regulation of both adhesion and cell morphology and cancer progression.  PODXL plays a role as an anti-adhesive molecule, which retains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. PODXL also functions as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligand which leads to rate increase of migration and cell-cell contacts in an integrin-dependent manner.