PIGF, PGF, PLGF-1.
PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It binds to receptor vegfr-1/flt1.
Placental Growth Factor-1 Human Recombinant produced in E.Coli is a non-glycosylated, disulfide-linked homodimer consisting of 2x132 amino acid polypeptide chains, having a total molecular mass of approximately 29.7kDa.
PLGF1 is purified by proprietary chromatographic techniques.
Sterile Filtered White lyophilized (freeze-dried) powder.
Lyophilized from a 0.2μm filtered concentrated solution in 10mM Sodium Phosphate pH 7.5.
It is recommended to reconstitute the lyophilized Placental Growth Factor-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized PLGF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Placental Growth Factor-1 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
MLPAVPPQQW ALSAGNGSSE VEVVPFQEVW GRSYCRALER LVDVVSEYPS EVEHMFSPSC VSLLRCTGCC GDENLHCVPV ETANVTMQLL KIRSGDRPSY VELTFSQHVR CECRPLREKM KPERCGDAVP RR.
Determined by its ability to chemoattract human monocytes of using a concentration range of 1.0-10.0 ng/ml.