Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Allergen Api m I, Api m 1.
Bee venom phospholipase A2 (PLA) is the main allergen in the bee sting allergy. Bee venom phospholipase A2 (BV-PLA2) is a hydrolytic enzyme which specifically cleaves the sn-2 acyl bond of phospholipids at the lipid/water interface. BV-PLA2 is a 14–16-kDa glycoprotein, consisting of 134 amino acids and displaying a single carbohydrate side chain at the residue Asn13. It is also held to be responsible for some systemic anaphylactic reactions in bee venom sensitized individuals. BV-PLA2 presents 3 peptide and a glycopeptide T cell epitopes, which are recognized by both allergic and non-allergic bee venom sensitized subjects. PLA is able to elicit both IgE mediated allergy and normal immunity to bee sting which usually is associated with high affinity IgG4 anti-PLA antibodies.
The E.Coli derived recombinant protein contains phospholipase P00630 bee venom protein epitopes, 26-162 amino acids. Phospholipase A2 P00630 Bee Venom is fused to a 6xHis tag at C-terminus
Purified by proprietary chromatographic technique.
Protein is >90% pure as determined by 10% SDS-PAGE.
1XPBS pH 7.2 and 50% glycerol.
Phospholipase A2 although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.