Pen a 1.0101 is a Tropomyosin and the only major allergen in brown shrimp Penaeus aztecus. The panallergen is highly conserved and soluble muscle protein. Pen a 1.0101 is has an alpha-helical homodimeric, coiled-coil structure.
Recombinant Tropomyosin Pen a 1.0101 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 34kDa.
Pen a 1.0101 is expressed with a 10xHis tag and purified by proprietary chromatographic techniques.
Sf9 insect cells.
Pen a 1.0101 is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl and 20% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Greater than 80.0% as determined by SDS-PAGE.
1. Binds IgE-type human antibodies.
2. Immunodot test with positive/negative samples
Tested by LAL (Limulus Amoebocyte Lysate) chromogenic endotoxin assay.