Nucleosome core proteins are a group of proteins which are necessary for DNA packaging in eukaryotes. Every core contains an octamer with 2 copies of each histone 2A, 2B, 3 and 4 around which an approx. 140 bp section of DNA is wound twice.
Nucleosome Bovine shows multiple bands between 14-18kDa and is purified from bovine tissues by proprietary protein-chemical techniques.
Nucleosome Bovine is supplied in 20mM HEPES buffer pH-7.5, 500mM NaCl, 1mM NaCl and 20% glycerol.
Avoid multiple freeze-thaw cycles.
Greater than 95.0% as determined by SDS-PAGE.
0.2-0.5 μg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.
1. Binds IgG type human auto antibodies.
2. Checkerboard analysis of positive/negative samples.