Neuritin 1, NRN1, NRN, dJ380B8.2, Neuritin.
Neuritin (NRN1) is a neurotrophic factor, which is expressed in response to induction of neuronal activity by NGF, BDNF, NT3 and other neural stimulators. NRN1 is expressed principally in postmitotic-differentiating neurons of the developing nervous system and in neuronal structures related to synaptic plasticity in the adult nervous system. NRN1 functions as a molecular mediator of neurite outgrowth, neuronal survival, and synaptic maturation.
Neuritin-1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Ala28-Gly116) containing 99 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 11.02kDa.
Filtered White lyophilized (freeze-dried) powder.
NRN1 was filtered (0.4 µm) and lyophilized in 20mM Tris buffer, 50mM NaCl, pH 8.0, 1% (w/v) Sucrose and 4% (w/v) Mannitol.
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. NRN1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.
Amino acid sequence
MKHHHHHHASAGKCDAVFKG FSDCLLKLGD SMANYPQGLD DKTNIKTVCT YWEDFHSCTV TALTDCQEGA KDMWDKLRKE SKNLNIQGSL FELCGSGNG.
Greater than 95.0% as determined by SDS-PAGE.
Safety Data Sheet
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.