Ag85

Ag85

Ag85

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  • View Data Sheet

    Name :

    Ag85B

    Description:

    Mycobacterium Tuberculosis major secretory protein Antigen 85B Recombinant

    Antigen 85-B, 85B, Extracellular alpha-antigen, Antigen 85 complex B, Ag85B, Mycolyl transferase 85B, EC 2.3.1.-, Fibronectin-binding protein B, 30 kDa extracellular protein, fbpB, A85B, Major Secretory Protein Antigen 85B.

    Product # :

    PRO-589

    Price :

    Quantity :

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    Shipped at Room temp

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    • description
    • source
    • formulation
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    Description

    Ag85B Recombinant His-Tag fusion protein produced in E.Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 30kDa.

    Source

    Escherichia Coli.

    Formulation

    Lyophilized with no additives.

    Purity

    Greater than 90.0% as determined by SDS-PAGE.

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    • Introduction

      Antigen 85B Mycobacterium Tuberculosis-is the most abundant protein exposed by M. Tuberculosis, as well as a potent immunoprotective antigen and a leading drug target. Ag85 induces strong T-cell proliferation and IFN-g secretion in most healthy individuals exposed to M. tuberculosis, in BCG-vaccinated mice and humans, whereas the antibody against Ag85 are more prevalent in active tuberculosis patients with decreased cellular immune response.

    • Synonyms

      Antigen 85-B, 85B, Extracellular alpha-antigen, Antigen 85 complex B, Ag85B, Mycolyl transferase 85B, EC 2.3.1.-, Fibronectin-binding protein B, 30 kDa extracellular protein, fbpB, A85B, Major Secretory Protein Antigen 85B.

    • Physical Appearance

      Sterile Filtered and lyophilized, though might appear as a solution as a result of the glycerol content.

    • Stability

      Ag85B although stable room temperature for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

    • Solubility

      It is recommended to reconstitute the lyophilized Antigen-85B in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    • Amino Acid Sequence

      MRGSHHHHHHFSRPGLPVEYLQVPSPSMGRDIKVQFQSGGNNSPAVYLLDGLRAQ
      DDYNGWDINTPAFEWYYQSGLSIVMPVGGQSSFYSDWYSPACGKAGCQTYKWETF
      LTSELPQWLSANRAVKPTGSAAIGLSMAGSSAMILAAYHPQQFIYAGSLSALLDP
      SQGMGPSLIGLAMGDAGGYKAADMWGPSSDPAWERNDPTQQIPKLVANNTRLWVY
      CGNGTPNELGGANIPAEFLENFVRSSNLKFQDAYNAAGGHNAVFNFPPNGTHSWE
      YWGAQLNAMKGDLQSSLGAG.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Ag85B
  • View Data Sheet

    Name :

    Ag85A

    Description:

    Mycobacterium Tuberculosis major secretory protein Antigen 85A Recombinant

    Ronectin-binding protein A, Mycolyl transferase 85A.

    Product # :

    PRO-1076

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    Quantity :

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    • description
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    Description

    Recombinant Mycobacterium tuberculosis Ag85A (43-338 a.a) produced in Hi-5 cells is a single, glycosylated polypeptide chain having a molecular mass of 32.8kDa (305 a.a in total).Antigen 85A is fused to a 6 amino acid His tag at C-terminus and purified by conventional chromatographic techniques.

    Source

    Baculovirus

    Formulation

    The Ag85A solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.

    Purity

    Greater than 95% as determined by SDS-PAGE.

    More Info

    • Introduction

      Antigen 85A is a member of the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex have mycolyltransferase activity and catalyze the synthesis of the very rich glycolipid of the mycobacterial cell wall, the cord factor (trehalose 6,6'-dimycolate, TDM). The cord factor is vital for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6'-monomycolate (TMM) by Antigen 85A.

    • Synonyms

      Ronectin-binding protein A, Mycolyl transferase 85A.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAH HHHHH.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Ag85A

About Ag85 / Mycobacterium Tuberculosis Major Secondary Protein Ag85 :

Mycobacterium Tuberculosis is a bacterium that is most well-known for causing tuberculosis (TB), an infection that primarily affects the lungs. Research into this disease has brought attention to the Mycobacterium Tuberculosis major secretory protein Antigen 85A, otherwise known as Ag85A.
The proteins of Antigen 85, including Ag85A, are the most common proteins found in the bacterium culture fluid and have a crystalline structure, although each of them is unique and therefore more suited for certain tasks.

Ag85 Mechanism
They cause the bacteria to be attracted to fibronectin, an adhesive glycoprotein that helps the bacteria to attach itself to alveolar macrophages, which in turn colonize the lungs of humans or animals. This allows the bacterium to find a home in the lungs.

Ag85 Function
A85 proteins also help to maintain the structural integrity of the cell wall. They do this by catalyzing the transference of mycolic acids to arabinogalactan, and by synthesizing alpha-trehalose dimycolate (TDM). Both arabinogalactan and TDM are vital for maintaining cell wall integrity.
Ag85A is used to generate TB vaccines, such as the BCG (bacillus Calmette-Guérin) vaccine. When used in a vaccine, it causes an immune response by inducing strong T-cell proliferation in the subject. Also, as the Ag85 proteins make up so much of the bacterium culture fluid, they make an even more attractive target for further research and potential vaccine development. This is important as, while the BCG vaccine has been effective in many cases, some strains and presentations of TB have proved resistant.