- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
PRO-2583
Synonyms
Meteorin-like protein, Subfatin, METRNL.
Introduction
METRNL or Meteorin-like protein is a hormone, released after physical activity (in skeletal muscle) or exposure to cold (in adipose tissue), that induces energy rate. METRNL stimulates energy rate correlate with white fat browning and raise glucose tolerance. The protein stimulates different types of immune cells to enter the fat tissue and start their prothermogenic actions.
Description
METRNL Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 46-311) containing 276 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 31.2kDa (calculated).
Source
Escherichia Coli.
Physical Appearance
Filtered White lyophilized (freeze-dried) powder.
Formulation
METRNL filtered (0.4 µm) and lyophilized from 0.5mg/ml in 50mM acetate buffer, 5% trehalose (w/v) and 1mM DTT, pH 4.
Solubility
It is recommended to add 0.1M acetate buffer, pH 4 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely.
Stability
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MKHHHHHHAS QYSSDRCSWK GSGLTHEAHR KEVEQVYLRC AAGAVEWMYP TGALIVNLRP NTFSPARHLT VCIRSFTDSS GANIYLEKTG ELRLLVPDGD GRPGRVQCFG LEQGGLFVEA TPQQDIGRRT TGFQYELVRR HRASDLHELS APCRPCSDTE VLLAVCTSDF AVRGSIQQVT HEPERQDSAI HLRVSRLYRQ KSRVFEPVPE GDGHWQGRVR TLLECGVRPG HGDFLFTGHM HFGEARLGCA PRFKDFQRMY RDAQERGLNP CEVGTD.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.