CCL8 Human

CCL8 Human

Chemokines

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  • Name :

    CCL8 Human

    Description:

    Monocyte Chemotactic Protein-2 Human Recombinant (CCL8)

    Small inducible cytokine A8, CCL8, Monocyte chemotactic protein 2, MCP-2, Monocyte chemoattractant protein 2, HC14, chemokine (C-C motif) ligand 8, MCP2, SCYA8, SCYA10.

    Product # :

    CHM-316

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    Shipped at Room temp

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  • description
  • source
  • formulation
  • purity
  • biological activity
  • More Info

Description

Monocyte Chemotactic Protein-2 Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 8904 Dalton. The MCP2 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity

The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human THP-1 cells. Significant calcium mobilization is observed with 500ng/mL of recombinant human MCP-2. Human MCP-2 also induces dose dependent chemotaxis of human THP-1 cells with an ED50=30-100 ng/mL  corresponding to a Specific Activity of 10,000-33,334IU/mg.

More Info

  • Introduction

    Chemokine (C-C motif) ligand 8 (CCL8) is a small cytokine belonging to the CC chemokine family that was once called monocyte chemotactic protein-2 (MCP-2). The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. MCP-2 is chemotactic for and activates a many different immune cells, including mast cells, eosinophils and basophils, (that are implicated in allergic responses), and monocytes, T cells, and NK cells that are involved in the inflammatory response. CCL8 elicits its effects by binding to several different cell surface receptors called chemokine receptors. These receptors include CCR1, CCR2B and CCR5.

  • Synonyms

    Small inducible cytokine A8, CCL8, Monocyte chemotactic protein 2, MCP-2, Monocyte chemoattractant protein 2, HC14, chemokine (C-C motif) ligand 8, MCP2, SCYA8, SCYA10.

  • Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

  • Stability

    Lyophilized MCP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL8should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

  • Solubility

    It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-2in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

  • Amino Acid Sequence

    The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ser-Val.

  • Background

    What is the molecular weight/Mw of CCL8 HUMAN Protein?
    CCL8 HUMAN Protein has a total Mw of 8.9kDa.

    What is the source or expression system of CCL8 HUMAN Protein?
    Escherichia Coli.

    What is the Purity of CCL8 HUMAN Protein?
    CCL8 HUMAN Protein is >95% pure as determined by SDS-PAGE.

    What is the Biological Activity of CCL8 HUMAN Protein?
    The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human THP-1 cells. Significant calcium mobilization is observed with 500ng/mL of recombinant human MCP-2. Human MCP-2 also induces dose dependent chemotaxis of human THP-1 cells with an ED50=30-100 ng/mL corresponding to a Specific Activity of 10,000-33,334IU/mg.

    What is the amino acid sequence of CCL8 HUMAN Protein?
    The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ser-Val.

    What applications can CCL8 HUMAN Protein be used in?
    CCL8 HUMAN Protein can probably be used in western blot, ELISA and Lateral Flow.

    What is the endotoxin level for CCL8 HUMAN Protein?
    The endotoxin level is minimal, CCL8 HUMAN Protein was purified using conventional chromatography techniques.

    Who supplies recombinant CCL8 HUMAN that is in stock and available to order?
    ProSpec has the protein in stock and available for immediate delivery.

    Where can I order bulk mg quantities of CCL8 HUMAN?
    ProSpec supplies bulk mg quantities at affordable prices that meets research and academic budgets.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Mcp 2 Human

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Frequently asked questions

1. Which company offers Recombinant Chemokines at competitive prices?

ProSpec offers vast array of Chemokines such as Eotaxin | Fractalkine | IL-8 | Rantes and much more at affordable descent prices to meet academic budgets
ProSpec provides High Purity Chemokines for 30 years, purity exhibits 95% and more as visualized by SDS-PAGE gel.
Chemokines are in stock for immediate delivery via FedEx which enables scientists and researchers to perform immediate experiments.
Chemokines are shipped globally using ProSpec’s custom tailored Styrofoam insulated box and premium ice packs that ensure maximum protein stability during transit to your laboratory.

2. Who can produce a custom Chemokine ?

ProSpec custom produces recombinant chemokines starting from Gene Design, Gene cloning, Expression Screening, Fermentation + protein expression, Cell harvesting + disruption, Protein recovery and purification to Q.C. formulation and filling, lyophilization is offered as an option.

3. What is the production time for a custom Chemokine?

The production time for a custom recombinant chemokine protein may vary between 5-7 weeks.

4. Where can i find biologically active Chemokines?

ProSpec offers biologically functional Chemokines such as SDF1A | Platelet Factor-4 | MCP1 which are rigorously tested in-vitro to provide researchers world wide BioActive Chemokines.

5. What is the difference between CC, CXC, CX3C, and XC chemokines?

Chemokines are a superfamily of small, secreted signaling proteins that direct the migration of immune cells, they are classified into 4 subfamilies based on the spacing of the cysteine residues near their N-terminus. CC and CXC are the largest families, while CX3C and XC contain fewer members. You can find CXC chemokines and CC chemokines at ProSpecBio such as cat# chm-348 Recombinant Human BCA-1/ BLC (CXCL13), cat# chm-001, Recombinant Human BRAK (CXCL14), cat#
chm-029, Recombinant Human CXCL16, cat# chm-331 Recombinant Human ENA78 (CXCL5) and more....

6. Which receptors bind to a specific chemokine?

Chemokines mainly bind G-protein-coupled receptors ( GPCR ), located on the surfaces of target cells. They match the structural classification of their ligands. Chemokines bind more than one receptor. For instance, CCL2 primarily binds CCR2, while CXCL12 binds CXCR4 and ACKR3. You can find CCL2 chemokine at ProSpecBio cat# chm-271, MCP1.