Non-specific lipid-transfer protein, LTP, MALD3.
MALD3 (a non-specific lipid transfer protein) is a major apple allergen. LTPs are found mainly in aerial plant organs such as leaves, seeds, flowers, and fruit. MALD3 is a heat-stable protein which is characterized by having resistance to pepsin hydrolysis.
Recombinant Non-Specific Lipid-Transfer Protein Mal d 3 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 12kDa.
MALD3 is expressed with a 6xHis tag and purified by proprietary chromatographic techniques.
Sf9 insect cells.
MALD3 is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl and 20% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Greater than 80.0% as determined by SDS-PAGE.
1. Binds IgE-type human antibodies.
2. Immunodot test with positive/negative samples
Tested by LAL (Limulus Amoebocyte Lysate) chromogenic endotoxin assay.