prospec

A2M Human

  • Name
  • Description
  • Pricings
  • Quantity
  • A2M Human
  • Macroglobulin Alpha-2 Human
  • Shipped at Room temp.

Catalogue number

PRO-551

Synonyms

Alpha-2-macroglobulin, Alpha-2-M, A2M, CPAMD5, FWP007, S863-7, alpha 2M, DKFZp779B086.

Introduction

Alpha-2 Macroglobulin is a serine proteases inhibitor, which inhibits coagulation by inactivating thrombin and Kallikrein, it inhibits fibrinolysis by inactivating plasmin and involved in transport. Alpha-2-Macroglobulin is a large plasma protein, which is produced by the liver, it’s composed of 4 identical subunits bound together by -S-S- bonds. A2M is able to inactivate many kinds of proteinases (including serine-, cysteine-, aspartic- and metalloproteinases). A2M has a 35 amino acid "bait" region in its structure. Proteinases bind and cleave the “bait” region become bound to A2M. Macrophage receptors recognize the proteinase-A2M complex and clear it from the system. A2M binds to and removes MMP-2 and MMP-9 (active forms of the gelatinase) from the circulation using scavenger receptors on the phagocytes. The levels of Alpha-2-macroglobulin are increased in nephrotic syndrome which is a condition where the kidneys start to leak out some of the smaller blood proteins. Due to its large size, A2-macroglobulin is retained in the bloodstream. Increased production of all proteins causes A2-macroglobulin concentration to increase. Chronic renal failure might lead to amyloid by alpha-2-macroglobulin. A2M is raised in cirrhosis, pregnancy and diabetes.

Description

Human Alpha-2 Macroglobulin is a tetrameric glycoprotein, produced in Human plasma and having a molecular mass of 725 kDa.

Source

Human Plasma.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a concentrated solution containing 5mM potassium phosphate buffer, pH 6.5 and 1:1 ratio (w/w) of Glycine.

Solubility

It is recommended to reconstitute the lyophilized A2M in sterile 18MΩ-cm H2O.

Stability

Lyophilized A2M protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution and filter sterilization A2M can be stored at 4°C for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Human Virus Test

Serum from each donor has been tested and found negative for antibody to HIV-1, HIV-2, HCV, HBSAG.

Purity

Greater than 95.0%.

Safety Data Sheet

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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