M6PR (242-277) Human

CD-MPR, MPR46, MPR 46, 46-KDa Mannose 6-Phosphate Receptor, MPR-46.

The M6PRHuman is created as a recombinant protein with a 4kda N-terminal fusion of His Tag. The M6PRHis-Tagged Fusion Protein, produced in E. coli, is a 9kDa protein containing 36 amino acid residues of the M6PRHuman, 242-277 amino acids.

Filtered White lyophilized (freeze-dried) powder.

Each mg was lyophilized with 1xPBS, 0.4% SDS and 4mM DTT.

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.

Store lyophilized M6PRat -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Greater than 90% as determined by SDS-PAGE.

SDS

 Mannose 6-phosphate receptors are membrane proteins which take part in the intracellular transport of lysosomal enzymes.

These receptors recognize and sort lysosomal hydrolases, facilitate their delivery to lysosomes via endocytic pathways.

M6PR binds to mannose 6-phosphate (M6P) residues on newly synthesized lysosomal enzymes in the Golgi apparatus, directing them to lysosomes.

After delivering enzymes to lysosomes, M6PRs are recycled back to the cell surface. This mechanism is critical for maintaining cellular homeostasis and efficient enzyme delivery.

M6PRs may also play a role in signaling pathways, influencing cellular responses to stress and metabolic changes.

Mutations or dysfunction in M6PRs can cause lysosomal storage disorders, emphasizing their importance in human health.

Recombinant M6PR has significant implications in therapeutic development, mainly in understanding lysosomal storage diseases and enhancing enzyme replacement therapies.