Lipase (EC 220.127.116.11) is a ubiquitous enzyme that catalyzes the hydrolysis of fats and oil. The Serratia marcescens lipase is recognized for its excellent enantioselectivity in biocatalytic hydrolysis of trans-3-(4-methoxyphynyl) glycidic acid methyl ester [(±)-MPGM] to produce (2R, 3S)-3-(4-methoxyphenyl) glycidic acid methyl ester [(-)-MPGM].
Recombinant Immobilized Serratia marcescens Lipase-A is expressed in E.Coli having a Mw of 65 kDa is purified by standard chromatography techniques.
Sterile Filtered lyophilized powder.
The protein was lyophilized without additives.
It is recommended to reconstitute the lyophilized Lipase-A in sterile 10%-50% DMSO, isopropyl ether, petroleum ether, ethanol, acetone and isopropanol.
Recombinant Lipase-A although stable at room temp for 1 week, should be stored desiccated below -18C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Unstable at higher than 50°C.
Higher activity can be reached in 10%-50% DMSO, isopropyl ether and petroleum ether, 10%-25% ethanol, acetone and isopropanol, 50% activity can be kept in 50% acetone and isopropanol, no activity detected in 50% ethanol.
Greater than 90% as determined by SDS-PAGE.
Lipase-A was assayed using p-nitrophenyl acetate ( pNPA) as a substrate.
One unit of lipase activity was defined as the amount of enzyme releasing 1.0 μmol of p-nitrophenol per minute.
580 units/mg powder.
Safety Data Sheet
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.