- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
PRO-1752
Synonyms
Lck-Interacting
Membrane Protein, Lck-Interacting Molecule, Lck-Interacting Transmembrane Adapter,
LP8067, dJ583P15.
Introduction
Lck-interacting
transmembrane adapter 1 precursor (LIME1) is a transmembrane adaptor protein
which is preferentially expressed in hemopoietic cells, mainly T cells. LIME1 mediates
TCR-dependent T cell activation, additionally LIME1 becomes tyrosine
phosphorylated after cross-linking of the CD4 or CD8 co receptors. The
inflammatory bowel disease is associated with LIME1.
Description
LIME1
Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 291 amino acids (28-295a.a) and having a molecular
mass of 30.8kDa.
LIME1 is fused to a 23 amino acid His-tag at N-terminus &
purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
LIME1 protein solution (0.5 mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze thaw cycles.
Purity
Greater than 80% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSCTACRRP EDAVAPRKRA RRQRARLQGS ATAAEASLLR RTHLCSLSKS
DTRLHELHRG PRSSRALRPA SMDLLRPHWL EVSRDITGPQ AAPSAFPHQE LPRALPAAAA TAGCAGLEAT
YSNVGLAALP GVSLAASPVV AEYARVQKRK GTHRSPQEPQ QGKTEVTPAA QVDVLYSRVC KPKRRDPGPT TDPLDPKGQG AILALAGDLA
YQTLPLRALD VDSGPLENVY ESIRELGDPA GRSSTCGAGT PPASSCPSLG RGWRPLPASL P
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.