The Leptin is purified by proprietary chromatographic techniques.
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
1. UV spectroscopy at 280 nm using the absorbency value of 0.878 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Leptin as a Reference Standard.
1.Title:Leptin and Amylin Act in an Additive Manner to Activate Overlapping Signaling Pathways in Peripheral Tissues In vitro and ex vivo studies in humans.
Publication:Published online before print September 24, 2010, doi: 10.2337/dc10-0518 Diabetes Care January 2011 vol. 34 no. 1 132-138 .
2.Title:The Mammalian Target of Rapamycin as Novel Central Regulator of Puberty Onset via Modulation of Hypothalamic Kiss1 System.
Publication: Published online before print September 4, 2009, doi: 10.1210/en.2009-0096 Endocrinology November 1, 2009 vol. 150 no. 11 5016-5026
3.Title:p38 Mitogen-Activated Protein Kinase and Liver X Receptor-? Mediate the Leptin Effect on Sterol Regulatory Element Binding Protein-1c Expression in Hepatic Stellate Cells.
Publication:Mol Med. 2012; 18(1): 10–18.
Publication: American Journal of Physiology-Endocrinology and Metabolism 298.2 (2010): E193-E201.