prospec

Insulin Human, Yeast

  • Name
  • Description
  • Pricings
  • Quantity
  • Insulin Human, Yeast
  • Insulin Human Recombinant, Yeast
  • Shipped at Room temp.

Catalogue number

CYT-614

Introduction

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Description

Insulin Human Recombinant produced in Yeast is a two chain, glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton. Zinc content was found to be 0.4%. Insulin is purified by proprietary chromatographic techniques.

Source

Saccharomyces cerevisiae.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized from 50mM Sodium Chloride solution.

Solubility

It is recommended to reconstitute the lyophilized Insulin in PBS pH-7.5.

Stability

Lyophilized Insulin although stable at room temperature, should be stored at 4°C. Upon reconstitution Insulin should be stored at -20°C to -80°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Biological Activity

27.5 units/mg.
Insulin has been evaluated in cell culture (Human Foreskin Fibroblasts).
The effective concentration range for use in defined media is 1-5ug/ml. However, the optimum concentration range for a particular application must be determined by the investigator.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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