Interleukin-5, IL-5, EDF, TRF, Eosinophil differentiation factor, T-cell replacing factor.
The protein encoded by IL5is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL5is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of IL5is reported to be related to asthma or hypereosinophilic syndromes. The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). Interleukin-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. Interleukin-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
IL5 Canine produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 119 amino acids (22-134 aa) and having a molecular mass of 13.9kDa.
IL5 is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Sf9, Baculovirus cells.
The IL5 solution (0.25mg/ml) contains 10% Glycerol and Phosphate-Buffered Saline (pH 7.4).
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Greater than 90.0% as determined by SDS-PAGE.
Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 15 ng/ml.
Amino acid sequence
VENPMNRLVA ETLTLLSTHR TWLIGDGNLM IPTPENKNHQ LCIKEVFQGI DTLKNQTAHG EAVDKLFQNL SLIKEHIERQ KKRCAGERWR VTKFLDYLQV FLGVINTEWT PESHHHHHH