- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
PRO-904
Synonyms
Hepatic leukemia factor, HLF.
Introduction
Hepatic leukemia factor (HLF) is a member of the bZIP family and the PAR (proline and acidic-rich) subfamily of transcription regulatory proteins. HLF binds DNA specifically as a homodimer or heterodimer with other PAR factors. HLF is highly expressed in the liver, with lower levels in the lung and kidney and functions as an effective trans-activator.
Description
HLF Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 315 amino acids (1-295 a.a.) and having a molecular mass of 35.3kDa.
HLF is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
HLF protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.2M NaCl and 0.1mM PMSF.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MEKMSRPLPL NPTFIPPPYG VLRSLLENPL KLPLHHEDAF SKDKDKEKKL DDESNSPTVP QSAFLGPTLW DKTLPYDGDT FQLEYMDLEE FLSENGIPPS PSQHDHSPHP PGLQPASSAA PSVMDLSSRA SAPLHPGIPS PNCMQSPIRP GQLLPANRNT PSPIDPDTIQ VPVGYEPDPA DLALSSIPGQ EMFDPRKRKF SEEELKPQPM IKKARKVFIP DDLKDDKYWA RRRKNNMAAK RSRDARRLKE NQIAIRASFL EKENSALRQE VADLRKELGK CKNILAKYEA RHGPL.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.