HIV-1 Nef is anessential factor for efficient viral replication and pathogenesis and therefore is produced shortly after virus infection. HIV-1 Nef is also facilitates virus replication and enhances virions infectivity. Nef exerts pleiotropic effects: decreases cell surface CD4 antigen by interacting with the Src family kinase LCK, down-modulates surface MHC-I molecules and protects the infected cell from apoptosis in order to keep it alive until the next virus generation is mature. Nef protein bypasses host T-cell signaling byinducing a trascriptional program almost identical to that of anti-CD3 cell activation.
Recombinant HIV-1 nef Biotin Labeled is a full length protein produced in E.coli and having a molecular mass of 27kDa. HIV-1 nef Biotin is purified by proprietary chromatographic technique.
Sterile Filtered clear solution.
HIV-1 nef Biotin protein solution containing PBS & 0.05%(v/v) glycerol.
HIV-1 nef Biotin although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles
Greater than 90.0% as determined by SDS-PAGE.
Western Blotting, SDS Page