prospec

HPSE Active

  • Name
  • Description
  • Pricings
  • Quantity
  • HPSE Active
  • Recombinant Human Heparanase-1 Active
  • Shipped with Ice Packs

Catalogue number

ENZ-1032

Shipping Conditions

Heparanase Active Enzyme is shipped frozen on dry ice unless stated otherwise by the customer.
Shipping fees to N. America and W. Europe is $250
Shipping fees to Asia, Australia and E. Europe is $350

Introduction

Heparanase is an endo β-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes (1,2,3). Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis (4,5). Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and a 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form (1,6). Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival (7,8).

Description

Heparanase Active Enzyme is produced in CHO cells.
The protein is purified by several orthogonal chromatography steps.

Formulation

Heparanase Active Enzyme is supplied in20mM Acetate buffer and 750mM NaCl pH 5.4.

Storage Procedures

Store at –80ºC, avoid repeated freeze-thaw cycles.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Specificity

Heparanase Active Enzyme is identified by Western blot analysis with polyclonal
rabbit anti-HPA1 antibodies as 2 subunits of 8-kDa and 50-kDa.

Biological Activity

The specific activity of Heparanase Active Enzyme in-house standard is about 0.7 Units (1 unit = 1 μmole of reducing ends of heparan sulfate substrate formed per minute per mg Heparanase Active Enzyme at 37°C). The enzymatic activity of each Heparanase Active Enzyme batch is comparable to the standard as determined by activity assay in which immobilized heparan, released due to heparanase activity, is quantified colorimetrically. Recommended reaction buffer: 20 mM Citrate Phosphate buffer, pH 5.4; 50mM NaCl; 1mM CaCl2.

References

1. Vlodavsky, I. et al.., Nat. Med. 5 (1999) 793–802.
2. Vlodavsky, I and Friedman, Y., J. Clin. Invest. 108 (2001) 341–347.
3. Parish, C.R. et al., Biochem. Biophys. Acta 1471 (2001) M99–M108.
4. Vlodasvsky, I. et al., Cancer Metastasis Rev. 9, (1990) 203-226.
5. Bashkin, P. et al.,. Biochemistry 28 (1989), 1737-1743.
6. Fairbanks, M.B. et al., J. Biol. Chem. 274 (1999) 29587– 29590.
7. Koliopanos, A.et al., Cancer Res. 61 (1992) 4655–4659.
8. Gohji, K. et al., Int. J. Cancer 95 (2001) 295–301.

Data Sheet

To view the FULL VERSION data sheet click Here

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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