HaeIII

site-specific DNA-methyltransferase, HaeIVRM.

HaeIII is a prokaryotic DNA that protects organisms from an unknown DNA. HaeIII’s recognition site is the GGCC nucleotide sequence which means it cleaves DNA at the site 5′-GG/CC-3.

E. coli that carries the HaeIII gene from Haemophilus aegypticus

20mM Tris-acetate (pH 7.9), 0.1mg/mL BSA, 10mM Magnesium Acetate and 50mM Potassium Acetate.

10mM Tris-HCl (pH 7.4), 1 mM DTT, 50% (v/v) glycerol, 0.1mM EDTA, 50 mM KCl and 200µg/ml BSA.

HaeIII although stable at 15°C for 1 week, should be stored below -18°C.

Please prevent freeze-thaw cycles.

Great than 95 % as estimated by SDS-PAGE analyses.

1 unit is defined as the amount of HaeIII required to digest 1 µg of lambda DNA in 1 hour at 37°C in 50 µL of recommended reaction buffer.

SDS

HaeIII cleaves the DNA at the positions where the GGCC sequence is found. The cleavage happens between the 2nd and the third nucleotides -G and C. The resulting DNA fragments are known as restriction fragments. HaeIII cuts both strands of DNA in the same location, creating restriction fragments with blunt ends.